I-Tris(hydroxymethyl)aminomethane

☑ I-Tris buffer ayisetshenziswa kakhulu nje kuphela njengesixazululi sama-nucleic acid namaprotheni, kodwa futhi inokusetshenziswa okuningi okubalulekile.I-Tris yayisetshenziselwa ukukhula kwekristalu yamaprotheni ngaphansi kwezimo ezihlukene ze-pH.
☑ Amandla e-ionic aphansi webhafa ye-Tris angasetshenziswa ukwenza umcu omaphakathi we-lamin kuma-C. elegans.
☑ I-Tris futhi ingenye yezingxenye eziyinhloko ze-protein electrophoresis buffer.
☑Ngaphezu kwalokho, i-Tris iyindawo emaphakathi yokulungiswa kwama-surfactants, ama-vulcanization accelerators nezinye izidakamizwa.I-Tris iphinde isetshenziswe njengezinga le-titration.
☑I-Trimethylaminomethane isetshenziswa kabanzi ku-acute metabolic kanye ne-accidemia yokuphefumula.Kuyi-alkaline buffer futhi inomphumela omuhle we-buffer ku-metabolic acidosis kanye nokusabela komsebenzi wama-enzyme.
☑I-Tris ivamise ukusetshenziswa njengebhafa yebhayoloji, futhi inani layo le-pH lingu-6.8, 7.4, 8.0, 8.8.Ifomula yayo yesakhiwo, inani le-pH lishintsha kakhulu ngezinga lokushisa.Ngokuvamile, inani le-pH lehla ngo-0.03 lapho izinga lokushisa likhuphuka ngedigri eyodwa.I-Tris isetshenziswa kakhulu ekulungiseleleni isisombululo se-buffer ku-biochemistry kanye nokuhlolwa kwebhayoloji yamangqamuzana.Isibonelo, kokubili amabhafa we-TAE kanye ne-tbe (asetshenziselwa ukuchithwa kwe-nucleic acid) avame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo adinga i-Tris.Ngenxa yokuthi iqukethe amaqembu amino, ingasabela nge-aldehydes.
☑ I-Tris isisekelo esibuthakathaka, futhi i-PKA yayo ingu-8.1 ekamelweni lokushisa (25 ℃).Ngokombono webhafa, ububanzi obusebenzayo bebhafa be-Tris buffer buphakathi kuka-7.0 no-9.2.Inani le-pH lesisombululo se-aqueous se-Tris licishe libe ngu-10.5.Ngokuvamile, i-hydrochloric acid iyengezwa ukuze kulungiswe inani le-pH enanini elifiswayo ukuze kutholwe isisombululo se-buffer ngaleli nani le-pH.Ngesikhathi esifanayo, kufanele sinake umphumela wezinga lokushisa ku-pKa ye-Tris.Ngenxa yokuthi i-Tris buffer iyisixazululo esibuthakathaka se-alkaline, i-DNA izokhishwa esixazululweni esinjalo ukuze kuthuthukiswe ukunyibilika kwayo.Abantu bavame ukwengeza i-EDTA ku-Tris hydrochloric acid buffer ukwenza i-"Te buffer", esetshenziselwa ukuqiniswa nokugcinwa kwe-DNA.Uma isisombululo se-asidi sokulungisa inani le-pH sithathelwa indawo i-acetic acid, i-"Tae buffer" (Tris / acetate / EDTA) iyatholakala, futhi "i-tbe buffer" (Tris / borate / EDTA) itholwa ngokufaka i-boric acid esikhundleni sayo. .Lawa mabhafa amabili ngokuvamile asetshenziswa ekuhlolweni kwe-nucleic acid electrophoresis.